In article <s-maloy-1111941704260001 at maloy3.life.uiuc.edu>, s-maloy at uiuc.edu
(Stanley Maloy) writes:
> Does anyone know where we could get a Tn10 derivative with Sp or Sm
> resistance (or any other selectable marker besides Tc, Km, Cm, Ap, or
> Hg)?
>> We want to do transposon mutagenesis in a Salmonella typhimurium strain
> with a Tn10 held chromosomal duplication and a Km resistant Mud fusion
> with a linked Tn10dCm insertion (for backcrosses) and saving Ap
> sensitivity (because we may want to bring in a Ap resistant plasmid for in
> vivo cloning of any interesting chromosomal mutants).
>> Thanks.
>> --
> Stanley Maloy
> Department of Microbiology
> University of Illinois
> 131 Burrill Hall, 407 S. Goodwin Ave
> Urbana, IL 61801
In Methods in Enzymology v. 204 Kleckner et al have an article that describes
Tn10 derived mini-Tn's. They have an element that contains a Kan gene that can
be used to generate Kan-translational fusions. It also has a selectable
erythromycin marker on it, it is available on an Ap-r pBR322 derivative.
Matthew L. Nilles (F0380903 at jaguar.csc.wsu.edu)
Dept. of Microbiology
Washington State University
Pullman, WA 99164
