In article <392jus$grl at ugle.unit.no> horn at alkymi.unit.no (Svein Horn) writes:
>Keywords:
>>I have analyzed some protein samples from E. choli.
>I used an anionic exchanger to separate the proteins ( used different NaCl -solut-
>ions 0.1,0.2,0.5 and 1.0 M).
>I also messured the protein consentration in each sample with Bio-Rad.
>>PROBLEM: The last sample( 1.0 M) gave a big top in the exchange experiment
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> ( Means high cons. of amino acids), But Bio-Rad showed a very low
> Protein cons.. I can't explain this. Anyone?
>> ( IN the 3 other samples the two methods agreed)
>>>>Svein
>
If you mean a large peak of material absorbing at 280 nm, then it is probably
nucleic acid - do a full spectrum and see where the absorbance max is. If at
ca. 260 nm, then almost certainly is nucleic acid. Should also be obvious by
SDS-PAGE if you have much protein or not.
Rob Solomon
Lost in CyberSpace
