In article <39ciaq$ogi at masala.cc.uh.edu>,
Michael Benedik <bchs1b at Jane.UH.EDU> wrote:
>>Does anyone know the survival of frozen perms stored in glycerol at -80
>after thawing and refreezing?
I don't think a single thaw-refreeze cycle will have a major detrimental
effect on culture survival. We have some glycerol-frozen preps which
have gone through several (three or four) of these cycles and have
maintained their viability.
However, if you're dealing with the last vial of an irreplaceable strain,
you might want to think twice about doing this... :^)
Gregg
