IUBio

Freeze-thawing permanent stocks

Michael Benedik bchs1b at Jane.UH.EDU
Fri Nov 4 00:54:34 EST 1994


Does anyone know the survival of frozen perms stored in glycerol at -80
after thawing and refreezing? I have a bunch of frozen perms which
I would like to transfer into new vials. It would be easiest to thaw
them and decant into the new vials. There are hundreds of stocks and
I would rather not have to regrow them all. These are mostly E. coli
strains.


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Michael Benedik						benedik at uh.edu
Department of Biochemical Sciences
University of Houston




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