In article <1994Jul19.140226.25820 at virginia.edu>,
Michael G. Kurilla <mgk2r at Virginia.EDU> wrote:
>After performing a literature search for PCR on Rickettsia, I
>have found several articles dealing with identification of the
>organism in ticks and methodologies to type various strains.
>>Is anyone aware of work in progess regarding isolation of the
>organism from blood of infected humans?
>If you really do mean isolation of the organism from from blood, the
technique has been well established for quite a few years. It basicly
involves serial dilution and passage through pathogen free embryonated
chicken eggs. For a reasonable review see the section on growth of
Rickettsia in:
Weiss, E. (1973) Bacteriological Reviews, 37:259-283.
For the original methods see the classic papers by Cox:
Cox, H. R. (1938) Pub. Health Rep. 53:2241-2247 and,
Cox, H. R. (1941) Science 94:339-403
Mike
--
LCDR M. Dobson | NetNews Admin net.usuhs.mil
Experimental Hematology Dept | dobson at net.usuhs.mil
Armed Forces Radiobiology |
Research Institute | I don't have enough rank to speak for DoD
