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Creating temperature sensitive mutants

ZOOMmmm zoommmm at aol.com
Fri Dec 23 04:36:16 EST 1994

Hi y'all!
  Soon, I'll be trying to create some temperature sensitive mutant
bacteria.  I'm hoping to use E. coli, but don't know yet which strain to
use.  Which strain is best suited in creating ts mutants?  Also, how good
is making ts mutants?  What are the best methods in creating ts mutants? 
I've heard they tend to revert back to normal.  I hope to have some
valuable incite before I start to make these mutants. 
Please e-mail to my address. Thanks.

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