I am looking for the ideal strain for general use in my lab and want to
know if it exists.
What I would really like is JM101 that is endA- ?
The markers I really want are: recA+, lacZM15, lacIq, endA-
We usually use the one step transformation method (PEG+DMSO) and want a
strain which transforms well with that method.
I have tried both JM105 and JM107, which should be fine for my use, but
both transform at least 10-fold lower efficiency using this method.
Anyone out there with our ideal strain?
Or alternately, does anyone have an endA- mutation linked to a
selectable marker which I could transduce in ?
Michael Benedik benedik at uh.edu
University of Houston
Houston, TX 77204-5934