I wish to use RAPD to fingerprint fluorescent pseudomonads. I have
been using REP-PCR (ala Versalovic) and have had great success. I now
want to expand the number of primers to exam more of the genome. Does
anyone have any experience with RAPD analysis of pseudomonads or
bacteria with similar G+C content and if so, do you have any
suggestions on a source of random primers. I know that there are
several sources of primers, I have looked into two, Operon and Genosys.
Operon supplies random primers with a G+C content of between 60 and
70%. Genosys' primers are specifically 60 or 70 or 80%, whatever you
want. Is it wise to initiate studies using a collection of primers
with a single percentage or a range of percentages? I would be
greatful for any comments/suggestions.