im posting this for a friend (really). he is attempting to express an
archeal gene in e. coli without much luck. we took a peek-see at the codon
usage and found (yikes) that 20% of his codons are used rarely in e. coli.
he is now thinking of using a eukaryotic expression system and is concerned
about glycosylation by, e.g., Pichia.
what are your suggestions for expression of archeal genes?