IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Expression of cloned archeal genes

Dan Diaz bl275 at cleveland.Freenet.Edu
Thu Dec 15 20:12:11 EST 1994



im posting this for a friend (really).  he is attempting to express an
archeal gene in e. coli without much luck.  we took a peek-see at the codon
usage and found (yikes) that 20% of his codons are used rarely in e. coli.

he is now thinking of using a eukaryotic expression system and is concerned
about glycosylation by, e.g., Pichia.

what are your suggestions for expression of archeal genes?



More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net