I have isolated an infectious agent growing on different cell
lines (from human fibroblasts to insect cell lines) with
induction of a cytopathic effect visable as swollen and rounded
cells before lysis. The cell culture medium is clear and looks
not infected. The agent is possible to transfer to a new culture
and a complete cytopathic effect appears within 5-7 days. Very
few infectious particles are possible to identify in the cell
culture medium and they are trapped in a 200 nm big membrane
filter. The agent grows at 16-50 centigrades. The growth is
inhibited by ribavirin, foscarnet (antiviral drugs) and also
acriflavine (an antibiotic for fungi infections). The
infectiousity is abolished by chloroform treatment. In
electronmicroscope on fixed human fibroblasts I have been unable
to identify any organism. In an infected cell culture I identify
an extra single stranded RNA band 2500 bases long when a RNA
preparation was done. No extra DNA band was visible. Gen-ProbeR
hybridization for Mycoplasma is negative.
I suspect an intracellular bacteria or fungi but a virus can
not be excluded completely.
My questions are:
Can anybody give a proposal how to distinguish between virus and
What different bacteria can you suspect?
What experiments can solve the problem? Antibiotics?
Jan ]ke Liljeqvist
Dept of Clinical Virology
University of Goteborg
Fax: +46.31.60 49 60
E-mail: ccugef at ccug.gu.se (or as above)