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growing condition to favour for complete R or SR phenotype

Lisa Demczuk ldemczuk at uoguelph.ca
Sun Dec 4 16:56:00 EST 1994


Raymond Tsang (rtsang at hpb.hwc.ca) wrote:
: I am looking for a condition to grow up Salmonella strains that favours 
: temporarily the production of only low numbers (e.g. one) or even no 
: O-specific side chains in their lipopolysaccharide.

Sounds like you want to make the strain rough.  Prolonged storage on 
media with a high sugar content would do the job.  However, since this 
roughness is determined chromosomally, it would be very difficult, if not 
impossible to get the strain smooth again.

 



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