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protein purification

Richard Seyler tad at bimcore.emory.edu
Thu Dec 1 13:24:11 EST 1994


In article 1 at lure.latrobe.edu.au, micjms at lure.latrobe.edu.au writes:
> Is there anyone out there that has used protein purification kits to purify
> prokaryotic proteins. I need to purify an E.coli protein to subsequently test
> for biochemical function. I would appreciate any information on the kits that
> have worked and those that haven't.

If you have the gene cloned and overexpression isn't a problem for cell growth,
you might try the NEB maltose binding protein fusion approach.  Folks in our lab,
and others in the department, have used this system with great success.
Good luck,  --Tad






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