In <CvCKv9.JG9 at magnus1.com>, maryr at magnus1.com (Bulletin board login) writes:
>I am looking for a protocol and/or reference for total RNA isolation
>from E. coli cells. Thanks, Mary
>
Try: Current Protocols in Molecular Biology - See Section 4
I like the rapid procedure for quick and dirty isolation. Otherwise do a
standard SDS lysis/CsCl quench followed by a CsCl cushion ultracentrifugation.
This method has as many variants as there are RNA researchers.
J.K. Spitznagel D.D.S., Ph.D.
