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RNA isolation from E.coli

Jack Spitznagel jks at daneel.utmem.edu
Tue Aug 30 20:41:08 EST 1994


In <CvCKv9.JG9 at magnus1.com>, maryr at magnus1.com (Bulletin board login) writes:
>I am looking for a protocol and/or reference for total RNA isolation
>from E. coli cells.  Thanks, Mary
>

Try: Current Protocols in Molecular Biology - See Section 4

I like the rapid procedure for quick and dirty isolation. Otherwise do a
standard SDS lysis/CsCl quench followed by a CsCl cushion ultracentrifugation.
This method has as many variants as there are RNA researchers.

J.K. Spitznagel D.D.S., Ph.D.



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