In article <32rmgc$ld6 at agate.berkeley.edu> micro at mendel.berkeley.edu
writes:
| Once we have the JC coordinator, we could start immediately. In the
| meantime people can pass on their suggestions for articles to me, and
I
| will pass them on in due course. Hope to see some stimulating
| discussion here shortly.
Anarchistically, I don't see why we shouldn't pass suggestions to you
in public
... and perhaps start discussion directly. The following is a rather
spectacularly surprising little paper:
@article{Tang1994,
author = "X. Tang
and Y. Nakata
and H.-O. Li
and M. Zhang
and H. Gao
and A. Fujita
and O. Sakatsume
and T. Ohta
and K. Yokoyama",
title = "The optimization of preparations of competent
cells for transformation of {{\em E. coli\/}}",
journal = "Nucl. Acids Res.",
volume = "22",
number = "14",
pages = "2857-2858",
comment = "1x10^8 transformants per ug DNA at O.D. 0.94",
year = "1994"}
The questions are: Why is this happening? What is the physiology?
Can it be
controlled? Is someone willing to do experiments? (I've thought of a
few
obvious ones.)
Tom Schneider
National Cancer Institute
Laboratory of Mathematical Biology
Frederick, Maryland 21702-1201
toms at ncifcrf.gov
Sincerely,
Martin Latterich
BIONET.MICROBIOLOGY Discussion Leader
micro at mendel.berkeley.edu
