In article <9407087763.AA776368509 at ccgate0.bms.com>, Stephen_W._Mamber_at_~PRIWFB05 at ccmail.bms.com writes:
> This microbiologist would like to know of any manufacturers
> of/references for nonisotopic methods for determining the inhibition
> of bacterial protein synthesis, DNA synthesis and/or RNA synthesis.
>> Thanks, SWM
>>mamber at synapse.bms.com>> OR
>> Stephen_W._Mamber_at_~PRIWFB05 at ccmail.bms.com>Stephen, the reference you are looking for is:
Brunschede, H., Dove, T.L., and Bremer, H. (1977) J. Bacteriol. 129, 1020-1033.
This gives a simple method for the non-isotopic determination of protein, RNA
and DNA, using absorbancies after various acid/alkali steps. It works! well
- I've used the RNA and protein part ;-)
Good luck,
Richard Heath
heath at mbcf.stjude.org
Department of Biochemistry
St. Jude Children's Research Hospital, Memphis, TN, 38101
