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protein concentration at 280 nm

Sudheer Sangeetham via methods%40net.bio.net (by sudheer.pbm07 from gmail.com)
Wed Mar 21 20:54:15 EST 2018


Hi

I wanted the check the concentration of purified recombinant protein at 280
nm which is at native form. To measure the concentration of it, do i need
to unfold the protein or native form would be fine., which one will give
best results. If any of the aromatic acids are not exposed to the solvent,
do they participate in absorbance?

Can someone reply.

Thank you

Regards
SUdheer


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