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Drop Dialysis of Ligations

shyamals86 from gmail.com via methods%40net.bio.net (by shyamals86 from gmail.com)
Wed Mar 14 17:24:31 EST 2018


On Thursday, September 14, 1995 at 3:00:00 AM UTC-4, Rich Kernan wrote:
> We frequently use 25 mm milipore filters floating in a petri dish of water to
> dialyze a few drops of ligation reaction prior to electroporation to avoid
> arcing.  These filters are fairly expensive and i was wondering if anyone could
> suggest an alternative or possibly a better method

Could you give me a protocol? I've ordered these membranes and have a hard time telling which is the  shiny side. Both look the same.

Shyamal


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