On Thursday, September 14, 1995 at 3:00:00 AM UTC-4, Rich Kernan wrote:
> We frequently use 25 mm milipore filters floating in a petri dish of water to
> dialyze a few drops of ligation reaction prior to electroporation to avoid
> arcing. These filters are fairly expensive and i was wondering if anyone could
> suggest an alternative or possibly a better method
Could you give me a protocol? I've ordered these membranes and have a hard time telling which is the shiny side. Both look the same.