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gc/ms for sphingosine

Wolfgang Schechinger via methods%40net.bio.net (by hubahopp from gmx.de)
Tue Nov 21 20:45:11 EST 2017


Hi Ed,

Have you checked with Google for "Sphingosine GC"? 3 hits in first 
place. Some derivatization involved apparently, but should be doable.

Regards

Wolfgang

On 22.11.2017 03:42, Ed Siefker wrote:
> I am interested in assaying sphingosine from cultured cells.  I have found
> many methods for LC-MS.  Unfortunately, my lab only has a GC-MS.
> I have been unable to find any discussion of GC-MS methods for sphingosine.
>
> ELISA methods exist for sphingosine-1-phosphate, and sphingosine can be
> phosphorylated easily enough.  I could subtract endogenous S1P from total
> S1P, but endogenous S1P is present at much higher concentrations than
> sphingosine, so I'm afraid my signal would be lost in the noise.
>
> Phosphorylation with 32P followed by TLC and scintillation counting is an
> option, but a last resort.
>
> Are there approaches I haven't considered here, or papers where these approaches
> have been successfully used?
>
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