I had found a simple centrifugal extrusion of the DNA from the gel to be gentler and less costly.
Hiranya S. Roychowdhury, Ph.D.
Department of Science
Division of Science, Engineering and Math
NMSU-Dona Ana Community College
575 527 7725 (office)
Curriculum & Instruction Committee
Human Anatomy and Physiology Society<http://www.hapsweb.org/>
From: methods-bounces from oat.bio.indiana.edu <methods-bounces from oat.bio.indiana.edu> on behalf of DK <dk from no.email.thankstospam.net>
Sent: Thursday, November 9, 2017 5:25:54 PM
To: methods from magpie.bio.indiana.edu
Subject: Re: Gel Solubilization Buffer Composition
In article <3a598d49-8251-49e6-a147-99e9bb3e5f0f from googlegroups.com>, angusrbishop from gmail.com wrote:
>On Saturday, 9 January 2010 18:42:04 UTC, wattne wrote:
>> On 9 Jan., 16:40, Jagadish Katam <jagadish... from hotmail.com> wrote:
>> > Hi,
>> > I am working on purifying the DNA by gel extraction procedure and are using
> the commercially available kits for this purpose. A yellow color gel
> solubilization buffer is used to dissolve the sliced gel.
>> > Here i would know the composition of this gel solubilization buffer.
>> > Any suggestions will be greatly appreciated.
>> > Thanks and Best Regards,
>> > Jag
>>>> Hi Jag!
>> Sounds like the buffer from QIAGEN, right? Why aren't you more
>> precise?? How should anyone help you if we don't even know what kind
>> of stuff you are using???
>> You can bet that the buffer composition is secret because otherwise
>> QIAGEN couldn't sell their kit anymore; what do you think? Try a
>> Google, and you will either find out or not. Why should we know the
>> exact formulation????
>> Thanks and Best Regards,
>>I found all the other replies to Jag's question helpful and constructive. Yours
> felt fairly malicious. Please refrain from comments like this in the future,
> its a waste of time and damages the community feeling of places like this.
I don't know about thje feelings but here is the composition:
5.5 M guanidine thiocyanate, 20 mM Tris-HCl, pH 6.6
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