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Slimy recombinant e.coli pellet

P J via methods%40net.bio.net (by pow.joshi from gmail.com)
Fri Nov 10 09:43:04 EST 2017

On a completely different note- DK, Wo, It's cool to see your name pop up
in my mail box. I've missed this community, I guess there haven't been too
many mol bio questions lately. So, it's cool to see you guys back! :)

On 9 November 2017 at 19:21, DK <dk from no.email.thankstospam.net> wrote:

> In article <mailman.110.1510233337.15473.methods from net.bio.net>, Megha
> Goyal <mgbiotec from gmail.com> wrote:
> >Dear All,
> >
> >We are doing fermentation for recombinant protein using E.coli culture.
> >Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after
> >IPTG Induction). But recently we observed that the cell pellet harvested
> >are of slimy consistency and they do not homogenise in the washing buffer
> >(tris-nacl-edta). We observe thread like consistency and no matter of
> >stirring it do we get a homogeneous suspension.
> >
> >Can someone guide me what is the reason for such phenomenon.
> Partial cell lysis. "Slime" = chromosomal DNA.
> DK
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