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Methods Digest, Vol 137, Issue 1

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Fri Nov 10 00:40:39 EST 2017


I am more than sure the culture is contaminated. Raise mother culture from
single well isolated clone.


On Nov 9, 2017 10:35 PM, <methods-request from oat.bio.indiana.edu> wrote:

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>    1. Slimy recombinant e.coli pellet (Megha Goyal)
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> Message: 1
> Date: Thu, 9 Nov 2017 11:41:15 +0530
> From: Megha Goyal <mgbiotec from gmail.com>
> Subject: Slimy recombinant e.coli pellet
> To: methods from magpie.bio.indiana.edu
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> Dear All,
>
> We are doing fermentation for recombinant protein using E.coli culture.
> Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after
> IPTG Induction). But recently we observed that the cell pellet harvested
> are of slimy consistency and they do not homogenise in the washing buffer
> (tris-nacl-edta). We observe thread like consistency and no matter of
> stirring it do we get a homogeneous suspension.
>
> Can someone guide me what is the reason for such phenomenon.
>
> Thanks
>
> megha
>
>
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