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Slimy recombinant e.coli pellet

Wolfgang Schechinger via methods%40net.bio.net (by hubahopp from gmx.de)
Thu Nov 9 10:13:58 EST 2017


Looks like contamination, Megha. Do you find your protein in the slime?

Regards,
Wo

On 09.11.2017 14:11, Megha Goyal wrote:
> Dear All,
>
> We are doing fermentation for recombinant protein using E.coli culture.
> Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after
> IPTG Induction). But recently we observed that the cell pellet harvested
> are of slimy consistency and they do not homogenise in the washing buffer
> (tris-nacl-edta). We observe thread like consistency and no matter of
> stirring it do we get a homogeneous suspension.
>
> Can someone guide me what is the reason for such phenomenon.
>
> Thanks
>
> megha
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