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kit recommendation for Baculovirus generation

Mike Autry via methods%40net.bio.net (by jma from ddt.umn.edu)
Mon Jun 22 13:02:11 EST 2015


Please provide recommendation for generating recombinant baculoviruses. I have used BaculoGold kit since the 1990s, but the kit is no longer available. I am unfamiliar with next-generation kits which use bacterial splicing of bacmid or Gateway plasmid shuttle. My expression plasmids contain AmpR, ori, polH promoter, and BV recombinant region ORF603 (eg, pVL1393, pAcSG2), but I do not have Gateway vectors. Cell line of choice is Sf21, but also use Sf9 and HiFive. Thanks in advance.

Mike Autry, PhD
Assistant Research Professor, U of MN

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