On Thursday, 12 April 2012 11:31:09 UTC+5:30, Edward Valera wrote:
> Hello, I don't know if you could solve the problem with NaeI.
> I'm having the same problem trying to digest a plasmid.
>> If you have any information I would really apreciate this
>> Edward Valera
I am nto sure if you are talking to me, but yes, I am still struggling with Nae1. Looks like it is a highlys ensitive enzyme and requires optimization. Try adding acetylated BSA - maybe .2 microlitres, to the reaction to stabiliize it . If I get any results, I iwll let you know. Please do the same if you find anythign!