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Whole cell extract gel samples for western blot

Alejandro Miguel Martin Dunn via methods%40net.bio.net (by alejandro.martin from cigb.edu.cu)
Tue Apr 28 06:53:13 EST 2015


Plain vortexing has worked for me in the past. I have also tried squirting the sample through a small bore needle (gauge 23) using an insulin syringe, and it works, but is messier.

Hope it helps!

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Phelan, Paul J.
Sent: Monday, April 27, 2015 2:45 PM
To: methods from magpie.bio.indiana.edu
Subject: Whole cell extract gel samples for western blot

Sometimes our gel samples (whole cell extracts) for western blots are very viscous.  What are the best methods for lysing small cell extracts well enough so that they don't float back out of the wells when loaded?

Thank you,

Paul Phelan
Tufts University Boston
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