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E coli BL21, DE3

Toufic El Arnaout via methods%40net.bio.net (by elarnaot from tcd.ie)
Tue Apr 1 06:48:37 EST 2014


Hello,
I have used both the pLys and standard BL21(DE3). I didn't find a
difference in the expression levels. Some people might have more experience
depends on applications or protein etc.
Here is the function of pLys in the strains of coli (from Internet):

"The pLysS plasmid carried by the BL21 Star(tm)(DE3)pLysS strain produces T7
lysozyme to reduce basal level expression of the gene of interest. pLysS
confers resistance to chloramphenicol (CamR) and contains the p15A origin.
This origin allows pLysS to be compatible with pUC- or pBR322-derived
plasmids."

So those cells can tolerate another antibiotic (chloram.) and the pLysS
reduces basal level expreesion of the gene of interest.

Toufic El Arnaout




On Fri, Mar 28, 2014 at 5:03 PM, <methods-request from oat.bio.indiana.edu>wrote:

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> Today's Topics:
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>    1. RE: E coli BL21?DE3 ? (Alejandro Miguel Martin Dunn)
>    2. RE: E coli BL21?DE3 ? (DK)
>    3. Re: Publishing Equipment Twaks? (Dr Engelbert Buxbaum)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 27 Mar 2014 08:10:46 -0400
> From: Alejandro Miguel Martin Dunn <alejandro.martin from cigb.edu.cu>
> Subject: RE: E coli BL21?DE3 ?
> To: "methods from magpie.bio.indiana.edu" <methods from magpie.bio.indiana.edu>
> Message-ID:
>         <A0221805023FCB449610B2ECF58BD3F87C5287520F from titan.cigb.edu.cu>
> Content-Type: text/plain; charset="iso-8859-7"
>
> It's probably 'lambda' in capital letters (quite similar to capital
> delta). DE3 is a lambda prophage, hence the name.
>
> Hope it helps,
> alejandro
>
> -----Original Message-----
> From: methods-bounces from oat.bio.indiana.edu [mailto:
> methods-bounces from oat.bio.indiana.edu] On Behalf Of WS
> Sent: Thursday, March 27, 2014 2:11 AM
> To: methods from magpie.bio.indiana.edu
> Subject: E coli BL21?DE3 ?
>
> Dear colleagues,
>
> While in search for BL21(DE3) & friends, I discovered only some vials
> labelled "BL21ÄDE3" and some labelled "BL21ÄDE3pLYSE". I suppose that's
> just a mis-spelling and thus exactly what I'm looking for, as google yields
> exactly 1 hit for "BL21ÄDE3" and Pubmed doesn't return any, also when I'm
> checking for"BL21Ä(DE3)".  And such a denomination doesn't make any sense
> to me, too, as DE3 is not part of the natural genotype.
>
> I'd appreciate any comments.
>
> Thanks!
>
> Wo
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
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>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 28 Mar 2014 02:43:41 GMT
> From: dk from no.email.thankstospam.net (DK)
> Subject: RE: E coli BL21?DE3 ?
> To: methods from net.bio.net
> Message-ID: <lh2nkt$cbb$1 from dont-email.me>
>
> In article <mailman.49.1395942643.2726.methods from net.bio.net>, Alejandro
> Miguel Martin Dunn <alejandro.martin from cigb.edu.cu> wrote:
> >It's probably 'lambda' in capital letters (quite similar to capital
> delta). DE3
> > is a lambda prophage, hence the name.
>
> My thought exactly. Particularly when combined with DE3pLysE, which is
> a certain to be (DE3) strain carrying pLys plasmid.
>
> DK
>
>
> >Hope it helps,
> >alejandro
> >
> >-----Original Message-----
> >From: methods-bounces from oat.bio.indiana.edu
> > [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of WS
> >Sent: Thursday, March 27, 2014 2:11 AM
> >To: methods from magpie.bio.indiana.edu
> >Subject: E coli BL21?DE3 ?
> >
> >Dear colleagues,
> >
> >While in search for BL21(DE3) & friends, I discovered only some vials
> labelled
> > "BL21ÄDE3" and some labelled "BL21ÄDE3pLYSE". I suppose that's just a
> > mis-spelling and thus exactly what I'm looking for, as google yields
> exactly 1
> > hit for "BL21ÄDE3" and Pubmed doesn't return any, also when I'm checking
> > for"BL21Ä(DE3)".  And such a denomination doesn't make any sense to me,
> too,
> > as DE3 is not part of the natural genotype.
> >
> >I'd appreciate any comments.
> >
> >Thanks!
> >
> >Wo
> >_______________________________________________
> >Methods mailing list
> >Methods from net.bio.net
> >http://www.bio.net/biomail/listinfo/methods
> >
> >
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 28 Mar 2014 16:36:12 +0100
> From: Dr Engelbert Buxbaum <engelbert_buxbaum from hotmail.com>
> Subject: Re: Publishing Equipment Twaks?
> To: methods from net.bio.net
> Message-ID: <MPG.2d9fb55477372089989691 from News.Individual.DE>
> Content-Type: text/plain; charset="us-ascii"
>
> In article <2fca5d4a-19ec-4fc6-8719-4e543af65d69 from googlegroups.com>,
> novalidaddress from nurfuerspam.de says...
> >
> > Dear Colleagues,
> >
> > from time to time I'm doctoring some lab equipment to make it better
> suit my needs. Is there any journal that would accept these tweaks in
> exchange for a few impact points?
>
>
> BioTechniques might be worth a try, or perhaps a short paper in Anal.
> Biochem. if you can show that your tweak is more generally useful.
>
>
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