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LiCl precipitation?

Hiranya Roychowdhury via methods%40net.bio.net (by hroychow from nmsu.edu)
Thu Mar 7 22:42:32 EST 2013


How do you know that you got RNA?  Alkaline lysis does not give you globs of RNA.


Hiranya S. Roychowdhury, Ph.D.
Associate Professor
Health & Public Services
NMSU-Dona Ana Community  College
575 527 7725 (office)

________________________________________
From: methods-bounces from oat.bio.indiana.edu [methods-bounces from oat.bio.indiana.edu] on behalf of Ed Siefker [ebs15242 from gmail.com]
Sent: Thursday, March 07, 2013 2:29 PM
To: methods from magpie.bio.indiana.edu
Subject: LiCl precipitation?

I did an alkaline lysis miniprep and ended up with gobs and gobs of RNA.
I added an equal volume of 4M LiCl, chilled at -20C for 40 minutes, and
spun at 16K Gs for 20 minutes.  I don't see a pellet.  What am I doing wrong?

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