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FFPE tissue rehydration - which xylene?

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Mon Feb 18 19:50:25 EST 2013


On Sunday, February 17, 2013 11:25:25 AM UTC-5, Gerchman wrote:
> Greetings all 
> 
> 
> 
> We are trying to rehydrate FFPE samples for protein
> 
> extraction (I know it is tricky) and the first on the protocol call for
> 
> wash with Xylene to remove the paraffin. The problem is I am not clear
> 
> about which xylene as there are at least 3 different isomers (orto, meta
> 
> and para). 
> 
> 
> 
> Anyone knows which one is used? 

"Histological grade" xylene(s) sold by major lab suppliers is described as a mixture of isomers.

Nick
.



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