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Does Taq accespt ATP as substrate?

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Sun Feb 17 15:32:29 EST 2013


Taq does not need ATP for its reaction unlike ligases.  Moreover, I doubt whether it has an ATP binding motif at all.  
Jay


-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of DK
Sent: Friday, February 15, 2013 7:53 PM
To: methods from magpie.bio.indiana.edu
Subject: Re: Does Taq accespt ATP as substrate?

In article <05d1f417-23b6-44fa-b0f7-bb0c48a1acda from googlegroups.com>, WS <novalidaddress from nurfuerspam.de> wrote:
>Dear experts,
>
>I am just curious if Taq would accept ATP (instead of dATP) when adding 3'
> overhangs to a PCR product. 

I have no real idea but I'd be really surprised to find that it does. I think that it's only specialized terminal deoxynucleotidyl transferases that are promiscuous like that.

DK



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