I too have found that the EcoRI sites one expects to find flanking a
cDNA insert are often non-functional. This proved to be the norm and
not the exception in my last experiments involving gt10. I was able
to subclone HindIII/BglII fragments into HindIII/BamHI cut vectors.
Good Luck!.
*********************************************************************
* * *
* Dan Laflamme * *
* Dept. of Biochemistry * *
* University of Maine * *
* Orono, Maine 04469 * *
* (207) 582-2818 * *
* * *
*********************************************************************
