I've Recently noticed an ad for Invitrogens PCR cloning kit
where they say that Taq always adds an extra A to the 3' end
of a fragment being amplified. Does anyone have any info (reference?)
on this i.e. is it True? I cant find any reference to this activity
in the PCR textbooks. I've successfully cloned lots of PCR products
in the past but as I put sites on the ends I've never looked for extra A's.
Apologies if you've read this before but my mail failed last time