We need to isolate *viable* human monocytes from whole blood, with >90%
purity, and highest possible yield, of course :-). In the past we have
used mono-poly resolving media (Flow Labs), which gives two bands, one
containing polynuclear cells, the other containing mononukes. We need to
remove the lymphocytes (we have also had a slight problem with some pmn
contamination in the mono layer - poly layer, however, has been quite
All suggestions warmly welcomed. Email or post.
Thanks in advance,
S A V E T H E W H A L E S , K I L L O U R U N B O R N ??
Daniel G. Sinclair - UNC Chapel Hill - Satisfied in Jesus. :-). Ahhhhhh.