In a recent message Johan de Boer (FS300512 at YUSOL) asks:
>I am trying to find a source for the following restriction
>enzyme:
>> SplI or PfuI or PspI
>>They are isoschizomers. If anyone knows of a company that
>sells one of these, would you please let me know?
If I look up in PC/Gene using the REDIT program (the
restriction enzyme editor) I find the following info:
>>RESTRICTION ENZYME DATA EDITOR.
>>Enzyme name
Spl I____Y
>Recognition site
CGTACG___________Y
>Cut offset: 1__ bases.
>>Isoschizomers.
>__________________________________Y
>Availability.
>Amersham._________________________Y
>
The (old) Amersham catalogue I have (1989) claims that
Amersham is the only distributor of Spl I. But PC/Gene
doesn't seem to know that Spl I has any isoschizomers ...
But the REDIT program of PC/Gene is a bit out of date ...
Maybe the next update ;-)
disclaimer: I am in no way, shape or form an employee
or a sales rep for IntelliGenetics or Amersham.
"And that's the truth Ruth"
(Do the right thing, Spike Lee)
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"Je cherche a comprendre" Jacques Monod
B.F. Francis Ouellette Bitnet (Netnorth): userBFFO at SFU
Internet: userBFFO at cc.sfu.caOuellett at whistler.sfu.ca
address: Dept of Biol. Sci., Simon Fraser University
Burnaby, BC, V5A 1S6, Canada, (604)291-3021
FAX: (604)291-3496
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PS. I realize that being from Canada it may be politically
unwise (and this be the wrong place for it) to have a
signature "en francais" but hey, pourquoi pas!
;-)