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Yeast protoplasts /lyticase

DBK at aberystwyth.ac.uk DBK at aberystwyth.ac.uk
Mon Dec 3 03:54:51 EST 1990

Does anyone know a good enzyme (and source!) for degrading yeast
(Saccharomyces cerevisiae) cell walls to make protoplasts? We have
had a go with Sigma lyticase (mixture of stuff from Arthrobacter), which
certainly lives up to its name (i.e. clobbers the protoplasts after making
them). Once upon a time there was helkicase from snails, which is supposed to
be good (not in Sigma catalogue). [that was helicase!]

All help *VERY* gratefully received.
Many thanks,
Douglas Kell.

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