If you work with restriction endonucleases alot, you might find this
little program I wrote useful.
It integrates enzyme/buffer activity information from several different
restriction endonuclease manufacturers into a database. With it, you
can select the enzymes you want to use in a multiple restriction enzyme
digest and have the program give you a list of buffers that will work
for all of them (saves a little time looking through catalogs and
The database covers four enzyme manufacturers: Boehringer-Mannheim,
Gibco BRL, New England Biolabs, and Stratagene. I should be able to add
a couple more soon.
The program is available as a java applet that anyone can use for free
via the internet. The site address is:
Any feed back would be greatly appreciated!