I have noticed a difference in the calcualted Isoelectric point
if the GCG program PEPStats is compared to a method available at Expasy
(6.6 vs. 7.2). This made me wonder how the IEP is calculated. I
always thoughth it could be predicted in a similar manner as the molecular
weight (that is from the sequence alone, without info about buffer or
what ever).
Any help appreciated that explains this discrepancy.
Kind regards,
Clemens Suter-Crazzolara, PhD
Kind regards,
Clemens Suter-Crazzolara, PhD
*************************************************************************
* For HUSAR, W2H & GCG visit: http://genome.dkfz-heidelberg.de *
*************************************************************************
* German German Cancer Research Center DKFZ European *
* EMBnet Dept. Molecular Biophysics (H0200) Data *
* node INF 280, 69120 Heidelberg, Germany Resource *
* - Genius - phone ++49 6221-4223-42 fax -33 - EDR - *
*************************************************************************
