Aidan Weatherill (Aweather at worf.molbiol.ox.ac.uk) wrote:
: Ok you answered with lightning speed last time, try this!
: I have 12 sequences that are fairly similar, I want to work out an enzyme
: digest that will differentiate between them without, and here is the trick,
: cutting anwhere in a number of unrelated sequences.
: ie I want to be able to find out the enzymes that cut in a number of
: sequences and use this information to design a digest that does NOT use any
: of these enzymes.
try CloneIt
http://locus.jouy.inra.fr/soft/cloneit/cloneit.html
it can compares two sequences from the Restriction Enzymes point of view..
_______________________________
| IN | OUT | IN | OUT |
_______________________________
| SEQ1 | SEQ2 |
___________________________________________________________
(...)
Van91I [CCANNNN^NTGG ] | 0 | 0 | 0 | 0 |
VspI [AT^TAAT ] | 1 | 0 | 2 | 3 |
XbaI [T^CTAGA ] | 0 | 0 | 1 | 0 |
XcmI [CCANNNNN^NNNNT] | 1 | 2 | 0 | 0 |
XhoI [C^TCGAG ] | 0 | 0 | 0 | 0 |
XhoII [R^GATCY ] | 0 | 0 | 2 | 6 |
XmaI [C^CCGGG ] | 1 | 0 | 1 | 0 |
XmnI [GAANN^NNTTC ] | 0 | 2 | 1 | 1 |*
___________________________________________________________
| IN | OUT | IN | OUT |
_______________________________
| SEQ1 | SEQ2 |
_______________________________
Hope this helps
--
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6098)o%::%o9 Pierre LINDENBAUM
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6o%::%o(8609 Institut National de la Recherche Agronomique
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CloneIt WWW Home Page:http://locus.jouy.inra.fr/soft/cloneit/cloneit.html
