gene resynthesis

Seth Crosby crosby at milbrandt.wustl.edu
Wed May 21 14:32:36 EST 1997

Hail netters!!
We have been working for sometime trying to express a soluble protein.  
Many vectors, many systems, many species of cells...no luck.  We have 
begun thinking of simply resynthesizing the entire gene.  It's about 
1500bp.  In the process we plan to optimize codons, get rid of hairpins, 
introduce convenient sites, alter (we hope) "mystery elements", etc.  Is 
there a good text/review that discusses the various considerations of 
gene resynthesis?  Anyone tried it?
Seth Crosby, MD
Abbott Labs

More information about the Info-gcg mailing list

Send comments to us at biosci-help [At] net.bio.net