Martin Stone writes:
>I am trying to design the sequences of oligonucleotides to use for
>synthesis and expression of a gene. Can somebody please help me with the
>following two issues.
>>(1) Does anybody have any suggestions about a codon usage table available
>in gcg that has worked well for gene expression in E. coli? I want to
>optimize the sequence of my DNA to get maximum expression levels.
FETCH the file ECOHIGH.COD Synthesized genes designed according to this
codon table usually work well.
>(2) Is there any way in gcg of getting a list of restriction sites that
>could potentially be introduced into a DNA sequence without changing the
>amino acid sequence of the protein encoded by the DNA.
Try MAP -SILent on a sequence file containing precisely the reading frame.
It will look for restriction sites with one-base mismatches which do not
alter the translation. See GENHELP MAP OPTIONAL_PARAMETERS.
Hope this helps.
Paul H. Roy
Departement de biochimie,FSG
Universite Laval
Quebec, QC G1K 7P4
CANADA
Internet: 2020000 at SAPHIR.ULAVAL.CA
or: PROY at RSVS.ULAVAL.CA
Bitnet: 2020000 at LAVALVX1
Phone: +1 418 654 2705
FAX: +1 418 654 2715
