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restriction sites and gene synthesis

Martin Stone mastone at indiana.edu
Thu Jan 25 18:13:23 EST 1996


I am trying to design the sequences of oligonucleotides to use for
synthesis and expression of a gene. Can somebody please help me with the
following two issues.

(1) Does anybody have any suggestions about a codon usage table available
in gcg that has worked well for gene expression in E. coli? I want to
optimize the sequence of my DNA to get maximum expression levels.

(2) Is there any way in gcg of getting a list of restriction sites that
could potentially be introduced into a DNA sequence without changing the
amino acid sequence of the protein encoded by the DNA.

Thanks

Martin Stone
Department of Chemistry
Indiana University
(812)855-6779
mastone at indiana.edu



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