Chris Barry wrote:
>> Hello,
> I have been using the CODONPREFERENCE and FRAME programs of GCG to
> determine the open reading frames and the start codons. Unfortunately,
> in the graphical output, it does not give the exact 3 base location of
> the start/stop codons. I have tried using a text editor of the output
> files to see if they would give me some clue... they were not obvious.
> Is there a way to determine these locations easily? Any advice would be
> greatly appreciated. Thanks in advance.
>> Chris
I thought I would share this solution to my problem if any of you are
interested.
1)Create a text file with these three lines in it
pattern file start_codon
..
Start_C 1 ATG 0
2)Save as start_codon.dat
3)run map in WPI using start_codon.dat as the enzyme data file instead
of the default dat file.
4)the output should give the locations of the start codons
