Chris Barry wrote:
>> I have been playing around with the plasmid map on GCG. Could someone
> tell me how I would go about:
>> 1) Digesting the insert and vector (will have stickey ends)
> 2) Ligating the insert into the vector
> 3) Displaying the new circular construct using the GCG plasmidmap
>> Thanks in advance for your time and patience.
Ok! I am getting desparate now. If anyone has a clue to how to digest a
sequence with one specific endonuclease, please let me know. I am using
the graphical user interface to gcg wpi.