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Vector & Insert???

Chris Barry chbarry at mackiller.llnl.gov
Fri Apr 5 16:28:07 EST 1996


Chris Barry wrote:
> 
> I have been playing around with the plasmid map on GCG. Could someone
> tell me how I would go about:
> 
> 1) Digesting the insert and vector (will have stickey ends)
> 2) Ligating the insert into the vector
> 3) Displaying the new circular construct using the GCG plasmidmap
> 
> Thanks in advance for your time and patience.
> 
> Chris

Ok! I am getting desparate now. If anyone has a clue to how to digest a
sequence with one specific endonuclease, please let me know. I am using
the graphical user interface to gcg wpi.

Chris



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