Good afternoon
I have the following question.
A user wants to assemble fragments of a sequence. He also have the "seed"
sequence which is a known sequence.
Giving the seed sequence and the fragments for assembly, he used the
assembly package. He includes his seed sequence because the fragment
does not necessairly overlap.
But the result contains the seed sequence inverted.
The user wants this sequence to be the master for the assembly and be on
the given sens.
Exemple of the work
Contig: xxxx
6 f1 +-->
5 f2 +--->
4 f3 <---+
3 f4 +-->
2 seed <------------------------------------+
C CONSENSUS <------------------------------------+
|----------|----------|----------|---------|---------|
0 600 1200 1800 2400
seed has be inverted.
What can we do to keep that one intact?
Thank you
Johanne Duhaime
IRCM
duhaimj at ircm.umontreal.ca
