prime with one fixed primer

poole poole at lifesci.ucsb.edu
Tue Jul 25 20:52:29 EST 1995

Is there any way to force the GCG Prime program to use a specific
primer on one end (say a forward primer you already have on hand) and
then choose an appropriate downstream primer? If I include the "fixed"
foward primer in a data file with the -PRI option, it uses only this
one primer and naturally doesn't find any suitable primer pairs. 

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