I'm planning a new project on the effect of HIV proteins on T cells.
I will need the reagents listed below, and would be grateful if anyone
could tell me where to get them:
1. cDNA or an engineered gene encoding HIV-1 gp120 (or gp160),
preferably for expression in Chinese hamster ovary cells,
although others or soluble gp120 protein also welcome.
2. Antibodies (or hybridoma) against HIV-1 gp120,
the HIV-1 tat protein and human CD26.
3. A plasmid to test HIV tat protein function in eukaryotic cells,
eg an LTR-Luciferase plasmid.
Thanks very much, and feel free to ask if I can provide anything
(including help) to you, I'm a former yeast geneticist-turned-
immunologist to give you an idea what might be available.
Klaus Lehnert, University of Auckland, Dept. Molecular Medicine,
Private Bag 92019, Auckland, New Zealand.
E-mail: mmm_klehnert at mednov1.auckland.ac.nz