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MAP routine ENZyme qualifier

Peter Rice rice at embl-heidelberg.de
Tue May 24 08:17:17 EST 1994


In article <01HC6TWV7M9S0018R8 at crcvms.unl.edu>, CSMITH at CRCVMS.UNL.EDU writes:
> I am running a LOT of RE digest analyses with the MAP routine, using the same
> set of 20 RE's. Inputing the abrevs for each of the enzymes either as the 
> /ENZyme qualifier or as prompted from within the routine has been a tedious
> task. To aleviate some of the manual work, I tried using a file list of the 
> enzymes with the enzyme qualifier ... /ENZyme=@enzyme.list, but to no avail.
> Is there a WORKABLE and/or BETTER way of inputing a list of RE's ?

This is one of the extensions included in the EGCG package. The MAPSELECT
program is a version of MAP that writes a new enzyme file. You can then
use this file with /ENZFILE=filename (note: it must be ENZFILE not ENZYME
as in your example, otherwise MAP ignores it) in a later run of MAP.

The EGCG programs are available by anonymous FTP for VMS systems from
ftp.embl-heidelberg.de in directory pub/software/vax/egcg (the older version
distributed with GCG VMS and Unix versions did not include MAPSELECT).

 -----------------------------------------------------------------------------
 Peter Rice, EMBL                             | Post: Computer Group
                                              |       European Molecular
 Internet:    Peter.Rice at EMBL-Heidelberg.DE   |            Biology Laboratory
                                              |       Postfach 10-2209
 Phone:   +49-6221-387247                     |       69012 Heidelberg
 Fax:     +49-6221-387306                     |       Germany



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