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MAP routine ENZyme qualifier

g78138201%vax9k.dnet at NCHUD1.NCHU.EDU.TW g78138201%vax9k.dnet at NCHUD1.NCHU.EDU.TW
Wed May 11 04:22:04 EST 1994



From:	NCHUD1::"BIOSCI-REQUEST at net.bio.net" 11-MAY-1994 15:43
To:	info-gcg at net.bio.net
CC:	
Subj:	Re: MAP routine ENZyme qualifier

In <01HC6TWV7M9S0018R8 at crcvms.unl.edu> CSMITH at CRCVMS.UNL.EDU writes:

> I am running a LOT of RE digest analyses with the MAP routine, using the same
> set of 20 RE's. Inputing the abrevs for each of the enzymes either as the 
> /ENZyme qualifier or as prompted from within the routine has been a tedious
> task. To aleviate some of the manual work, I tried using a file list of the 
> enzymes with the enzyme qualifier ... /ENZyme=@enzyme.list, but to no avail.
> Is there a WORKABLE and/or BETTER way of inputing a list of RE's ?

Christoph Gartmann reply a possible solution:

>$ FETCH ENZYME.DAT
>$ EDIT ENZYME.DAT      !Use your favourite editor, e.g. EDIT/TPU
> now remove all the enzymes you don't want from the list and save
>$ MAP...
>
>Map will now read your local ENZYME.DAT (the programm will tell you) and
>you may simply choose the default when you are prompted for the enzymes.

 Here is another solution: You may write your own enzyme.dat file.
 For example, if you write a file enz.dat like:

 ..
 ApaI    5  G_GGCC'C  -4 
 EcoRI   1  G'AATT_C   4
 EcoRV   3  GAT'ATC    0

 And run MAP

 GCG>map/dat=enz.dat
 
 GCG will read  your enz.dat file, and when you select "*"(all enzyme),
 there shall be only 3 enzymes selected.
 Hope it will help. 
 :)

 William Y. Wen
 Taiwan R.O.C
 g78138201%vax9k.dnet at nchud1.nchu.edu.tw



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