From: NCHUD1::"BIOSCI-REQUEST at net.bio.net" 11-MAY-1994 15:43
To: info-gcg at net.bio.net
CC:
Subj: Re: MAP routine ENZyme qualifier
In <01HC6TWV7M9S0018R8 at crcvms.unl.edu> CSMITH at CRCVMS.UNL.EDU writes:
> I am running a LOT of RE digest analyses with the MAP routine, using the same
> set of 20 RE's. Inputing the abrevs for each of the enzymes either as the
> /ENZyme qualifier or as prompted from within the routine has been a tedious
> task. To aleviate some of the manual work, I tried using a file list of the
> enzymes with the enzyme qualifier ... /ENZyme=@enzyme.list, but to no avail.
> Is there a WORKABLE and/or BETTER way of inputing a list of RE's ?
Christoph Gartmann reply a possible solution:
>$ FETCH ENZYME.DAT
>$ EDIT ENZYME.DAT !Use your favourite editor, e.g. EDIT/TPU
> now remove all the enzymes you don't want from the list and save
>$ MAP...
>>Map will now read your local ENZYME.DAT (the programm will tell you) and
>you may simply choose the default when you are prompted for the enzymes.
Here is another solution: You may write your own enzyme.dat file.
For example, if you write a file enz.dat like:
..
ApaI 5 G_GGCC'C -4
EcoRI 1 G'AATT_C 4
EcoRV 3 GAT'ATC 0
And run MAP
GCG>map/dat=enz.dat
GCG will read your enz.dat file, and when you select "*"(all enzyme),
there shall be only 3 enzymes selected.
Hope it will help.
:)
William Y. Wen
Taiwan R.O.C
g78138201%vax9k.dnet at nchud1.nchu.edu.tw
