Sticking sequences together

Jeroen Coppieters jecop at gengenp.rug.ac.be
Wed Mar 23 12:36:35 EST 1994

On Wed, 23 Mar 1994, A Brown wrote:

> This should be extremely simple, but unfortunately, all the instructions
> for the GCG package that we have seem to be written in sanscrit, and
> no-one has much of a clue what to do.
> All I want to do is to take a sequence of a vector (which I have the
> file for) and insert the sequence for a gene (which I also have the file
> for) in the correct R.E. site. 
>  I have tried everything from a simple text editor, to GELASSEMBLE and a
> whole pile of other programs that were jolly interesting, but didn't do
> what I wanted. 
>  Please could someone tell me how to do this simple task, before all my
> hair falls out.
> Many thanks
> A. L. Brown
from the help of gcg (genhelp)

     Assemble  makes  new  sequence  constructs  from pieces  of  existing
     sequences.  It concatenates the fragments you specify and writes them
     out as a new sequence file.  SeqEd is more powerful than Assemble for
     most applications.


     Assemble lets you choose as many segments as you please from existing
     sequences.   The ranges chosen can be from  either  strand.  Assemble
     puts all of the segments together into a new sequence file.

     Assemble supports  the  assembly of fragments from circular molecules
     by letting you define a range in the sequence that extends across the
     end and into  the beginning of a molecule.  The  terminal  bell rings
     when a circular range is chosen.

		jecop at gengenp.rug.ac.be
                    /     Jeroen Coppieters
     \ \   /\/\  /\/      Lab of Genetics
      \ \ / /\ \/ /\      University of Gent.
       \ /\/  \/\/  \    /\   AG    GC    C       Ledeganckstraat 35
                     \  /  \ T  A  G  A  A        B-9000 Gent (Belgium)
                      \/    A    AA    GA         32-9-2645189

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