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In <2hpmomINNaqn at newsstand.cit.cornell.edu> douglas writes:
> Regarding PCR primer design and GCG...
> Is there a way to search for PCR primers using any of the available GCG
> programs? I have heard that there was a program developed to use the RNA
> fold algorithms, but have not been able to access it.
Here we use
PRIMER: A Computer Program for Automatically Selecting PCR Primers
Version 0.5, May 1991
by Stephen E. Lincoln, Mark J. Daly, and Eric S. Lander
MIT Center for Genome Research and
Whitehead Institute for Biomedical Research
Nine Cambridge Center
Cambridge, Massachusetts 02142
in conjunction with
!*** ToPrimer **************************************************************
!*
!* translates a GCG sequence file into Primer sequence file
!* Old name: ToStaden
!* Copyright John Devereux 1983
!* Modified by Rodrigo Lopez 1991
!******************************************************************************
and a self-made DCL-command file (yes, on VMS; but it should be very simple
to convert the DCL into a UNIX script). All this together fits perfcetly into
the GCG environment and people are quite content with it.
Regards
Christoph Gartmann
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