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Charles Bailey bailey at hmivax.humgen.upenn.edu
Thu Apr 14 01:01:11 EST 1994


In article <31889FE5535F402967 at vxircm.IRCM.UMontreal.CA>, JOHANNE at IRCM.UMontreal.CA writes:
> The porblem is the following:
> one researcher wants to know if there is any program able to identify
> the Restriction fragments lenght polymorphism of any gene?
> I do not think that any GCG program does that. Does someone has any
> idea?

I'm not sure what you're asking here.  A restriction fragment length
polymorphism is a phenomenon which occurs over a population, not over
a single copy of a sequence.  There are programs in the GCG package
which will determine the length of restriction fragments for a given
sequence and set of restriction enzymes, and there are programs which
can be used to compare multiple sequences and highlight differences,
so you could in theory figure out whether a RFLP existed in a given
sequence by comparing that sequence from many individuals and noting
whether any positions at which substitutions were common would create
useful RFLPs.  This approach is much less efficient, however, than
just digesting DNA from several individuals with the restriction
enzymes of interest and looking for polymorphic bands on Southern
blots.

If I've completely misinterpreted your question, please feel free to
correct me.

                    Regards,
                    Charles Bailey

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